ATP synthase Specific Activity Microplate Assay Kit

Cat.No. :
Kit-0117
Product Overview :
Rapid multiplexing microplate kit is used to determine both the activity and quantity of ATP synthase (Complex V) in a Human or Rat sample. The ratio of the two measurements represents the enzyme"s specific activity. The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. Specifically, the conversion of ATP to ADP by ATP synthase is coupled to the oxidative reaction of NADH to NAD+. The formation of NAD+ results in a decrease in absorbance at 340 nm. Subsequently, in these same wells, the quantity of ATP synthase is determined by adding an ATP synthase specific antibody conjugated with alkaline phosphatase. This phosphatase changes the substrate pNPP from colorless to yellow (OD 405 nm) in a time dependent manner proportional to the amount of protein captured in the wells. Included for optimal performance of the assay are buffer, detergent, antibody, substrate, lipid mix and a 96-well microplate pre-coated with monoclonal antibody.
Storage :
Store Buffer, Detergent, Detector Antibody, 2500X AP Label, AP Buffer, and the microplate at 4°C. Store the Reagent Mix, AP Development Reagent and Lipid Mix at -20°C. For multiple experiments, the Reagent Mix and AP Development Reagent, and Lipid Mix may be aliquoted and stored at -80°C to minimize freezethaw cycles.
Size :
96 tests
Kit Components :
Buffer (Tube 1) 15 ml
Detergent 1 ml
Reagent Mix 20 ml
Detection Antibody (Tube A) 1 ml
Lipid Mix 6 ml
96 well microplate (12 strips) 1
2500X AP Label (Tube B) 0.012ml
AP Buffer (Tube 2) 20 ml
AP Development Reagent (Tube 3) 0.4 ml
Detection method :
Colorimetric
Compatible Sample Types :
Cell culture supernatant, Cell culture extracts, Tissue, Tissue Extracts, Cell Lysate
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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