Biotin Labeling Efficiency Assay Kit (HABA Method)

Cat.No. :

Kit-3970

Applications :

- Quality control of biotinylated antibodies and conjugates for immunoassay, IHC, and flow cytometry applications
- Assessment of biotinylation degree in labeled probes and detection reagents
- Optimization and screening of biotinylation reaction conditions
- Routine QC of biotin-labeled intermediates in diagnostic and research reagent manufacturing

Product Overview :

This kit provides a simple and rapid colorimetric method for evaluating biotin labeling efficiency of antibodies, proteins, and other amine-containing biomolecules. The HABA (4'-Hydroxyazobenzene-2-carboxylic acid) displacement assay enables straightforward estimation of the degree of biotinylation without requiring specialized instrumentation.

Features & Benefits :

- Simple protocol with minimal hands-on time and no need for complex equipment
- HABA-based colorimetric readout visible with standard spectrophotometers
- Rapid results suitable for screening multiple samples in parallel
- Cost-effective solution for routine biotinylation quality assessment