Caspase-2 Colorimetric Assay Kit

Cat.No. :

Kit-1119

Description :

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Colorimetric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.

Applications :

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Storage :

-20°C

Shipping :

Gel Pack

Kit Components :

Cell Lysis Buffer; 2X Reaction Buffer; VDVAD-pNA (4 mM); DTT (1 M); Dilution Buffer

Target Species :

Mammalian

Detection method :

Absorbance (400 or 405 nm)

Features & Benefits :

Simple one-step procedure; takes 1-2 hours;
Fast and convenient;
Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-2 activity.