Caspase-2 fluorometric assay Kit

Cat.No. :

Kit-0160

Product Overview :

The Caspase-2 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity.

Storage :

-20°C

Shipping :

Gel Pack

Size :

25 assays

Kit Components :

25ml Cell Lysis Buffer,
2ml 2X Reaction Buffer,
125µl VDVAD-AFC Substrate (1mM),
100µl DTT.

Target Species :

Mammalian

Detection method :

Fluorecence (400 nm excitation filter and 505 nm emission filter)