Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate IETD-pNA.
Description :
The FLICE/Caspase-8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD.
Storage :
Store kit at –20°C (Store Cell Lysis Buffer, 2X Reaction Buffer, and Dilution Buffer at 4°C after opening). All reagents are stable for at least 6 months.
Size :
25 tests
Handling :
Aliquot enough 2X Reaction Buffer for the number of assays to be performed. Add DTT to the 2X Reaction Buffer immediately before use (10 mM final concentration: add 10 µl of 1.0 M DTT stock per 1 ml of 2X Reaction Buffer). • After thawing, store Cell Lysis Buffer, 2X Reaction Buffer, and Dilution Buffer at 4°C. • Protect IETD-pNA from light.
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