Description :
Catalase (EC 1.11.1.6) is a ubiquitous antioxidant enzyme that is present in nearly all living organisms. It functions to catalyze the decomposition of hydrogen peroxide (H₂O₂) to water and oxygen. Catalase Assay Kit provides a highly sensitive, simple, direct and HTS-ready assay for measuring Catalase activity in biological samples. In the assay, catalase first reacts with H₂O₂ to produce water and oxygen, the unconverted H₂O₂ reacts with OxiRed probe to produce a product, which can be measured at 570 nm (Colorimetric method) or at Ex/Em=535/587nm (fluorometric method). Catalase activity is reversely proportional to the signal. The kit detects high pico-unit of catalase in samples.
Applications :
The kit detects high pico-unit of catalase in samples.
Storage :
Store kit at 4°C, protect from light. Warm the assay buffer to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.
Handling :
OxiRed Probe: Briefly warm to completely melt the DMSO solution. Store at 4°C, protected from light. Use within two months.
HRP: Dissolve with 220 µl Assay Buffer. Store at 4°C. Use within two months.
Positive Control Solution: Add 500 μl Assay Buffer to Positive Control. Aliquot and store at -20°C. Diluted Positive Control solution is stable for 2-3 days at 4°C & for 2 months at -20°C.
Note: Keep samples, HRP and Catalase on ice while in use.
Kit Components :
• Catalase Assay Buffer
• OxiRed Probe (in DMSO)
• HRP (lyophilized)
• H₂O₂ (3%; 0.88M)
• Stop Solution
• Catalase Positive Control (lyophilized)
Target Species :
Mammalian
Detection method :
Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Features & Benefits :
• Simple procedure; takes ~ less than 40 minutes
• Fast and convenient
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