Cathepsin L Inhibitor Screening Kit (Fluorometric)

Cat.No. :

Kit-2351

Product Overview :

Cathepsin L (CTSL, EC 3.4.22.15) is a lysosomal cysteine protease that is implicated in protein degradation, arthritis, apoptosis, and cancer. Cathepsin L Inhibitor Screening Kit utilizes the ability of active Cathepsin L to cleave the synthetic AFC-based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin L inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin L.

Applications :

Screen/study/characterize potential inhibitors of Cathepsin L

Storage :

-20°C

Shipping :

Gel Pack

Size :

100 assays

Kit Components :

CTSL Assay Buffer; CTSL Reagent; DTT; Cathepsin L (human, 20 mU); CTSL Substrate, Ac-FR-AFC (10 mM); CTSL Inhibitor (FF-FMK, 1 mM)

Detection method :

Fluorescence (Ex/Em 400/505 nm)

Features & Benefits :

Convenient & Rapid;
High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin L;
Cathepsin L inhibitor, FF-FMK is included in the kit