Product Overview :
Cell-Free Protein Expression Kit is designed for in vitro transcription and translation of target DNA to protein in a single reaction. This product contains an optimized E.coli extract containing T7 RNA polymerase for transcription and ribosome for translation. An optimized Master mix provides all other required components, including amino acids, rNTPs, and appropriate salts to express high-level production of recombinant proteins. This product contains reagents for expression of protein directly from a variety of DNA templates, includingT7 promoter and terminator as well as RBS (ribosomal binding site). This product can be used to express a wide range of target proteins and has the capacity for a yield of more than 300 µg/mL of protein within 3 hours
Applications :
This product can be used to express a wide range of target proteins and has the capacity for a yield of more than 300 µg/mL of protein within 3 hours.
Storage :
Store kit at -20°C ~ - 70°C.
Size :
45 µL x 24 reactions
Kit Components :
Master mix: 0.54 mL × 1 tube;
E.coli extract: 0.3 mL × 1 tube;
DEPC DW: 1 mL × 1 tube;
Positive Control DNA: 1 tube.
Features & Benefits :
High-speed: synthesize your target protein quickly (within 3 hours) and economically
Easy-to-Use: includes all necessary components for transcription and translation
Flexible: synthesize proteins from various DNA templates.
synthesize proteins of toxic proteins
incorporate non-natural amino acids
use additives such as metal ions, detergents, cofactors, binding partners
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