Product Overview :
CHIA Assay Kit (Fluorometric) is used for the quantitative measurement of acidic mammalian chitinase (AMCase) activity in a culture supernatant of stimulated pulmonary epithelial cells, several tissue extracts and other biological samples.
Description :
The glycosyl hydrolase family is quite large, with over 100 families. Acidic mammalian chitinase (AMCase) and chitotriosidase-1 are enzymatically active true chitinases in a member of the glycosyl hydrolase-18 family, identified by EC 3.2.1.14. In mammals, only these two enzymes cleave N-acetyl-beta-D-glucosamine (1->4)-beta linkages in chitodextrins and chitin, the second most abundant biopolymer that can be found in the cell walls of fungi, micro-filarial sheaths of helminths, and exoskeletons of insects and crustaceans. Other memberincluded in this family are chitinase-like molecules, e.g. YKL-40/Chitinase-3-like-1 and YKL-39/chitinase-3-like-2, that are thought to lack the ability to hydrolyze the chitin linkages, but retain chitin-binding activity and act more like connective tissue proteins, and immediate early gene products.
Applications :
CHIA Assay Kit (Fluorometric) is used for the quantitative measurement of acidic mammalian chitinase (AMCase) activity in a culture supernatant of stimulated pulmonary epithelial cells, several tissue extracts and other biological samples.
Usage :
For research use only (RUO)
Storage :
• Upon receipt store "Recombinant AMCase" at -70°C and all other components below -20°C.• Don"t expose reagents to excessive light.
Kit Components :
10X AMCase Assay Buffer (pH 2.0) 1.0 mL x 210X Chitinase Assay Buffer (pH 4.0) 1.0 mL x 210X Fluoro-Substrate (200 µM 4-MU-chitotrioside*) 1.0 mL x 110X Recombinant AMCase ** 1.0 mL x 1Enzyme Dilution Buffer 1.0 mL x 24-Methylumbelliferone Standard (100 µM) 200 µL x 1 * 4-Methylumbelliferyl-β-D-N,N",N"-triacetylchitotrioside** Recombinant human AMCase expressed in HEK293 cells.
Detection method :
Fluorometric
Compatible Sample Types :
Culture Supernatant of Stimulated Pulmonary Epithelial Cell, Several Tissue Extract, Other Biological Sample
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