Product Overview :
This kit uses Blue, our chromogenic HRP substrate that is much more sensitive to both H2O2 and peroxidase than other chromogenic peroxidase substrates such as TMB, ABTS, OPD and K-Blue. Blue generates a highly absorptive material that has maximum absorption of 664 nm. This near infrared absorption minimizes the background absorption often caused by the auto-absorption of biological samples. The kit provides an optimized "mix and read" assay protocol that is compatible with HTS liquid handling instruments. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by an absorbance microplate reader at 664±5 nm.
Description :
Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques; Northern, Southern and Western blot analyses. Due to its small size, it rarely causes steric hindrance problem with the antibody/antigen complex formation. It is usually conjugated to an antibody in a 4:1 ratio. Additionally, HRP is inexpensive compared to other labeling enzymes. The major disadvantage associated with peroxidase is their low tolerance to many preservatives such as sodium azide that inactivates peroxidase activity even at low concentration.
Storage :
Keep in freezer and avoid exposure to light.
Kit Components :
Component A: Blue Peroxidase Substrate 1 vial
Component B: H2O2 1 vial (3% stabilized solution, 200 μL)
Component C: Assay Buffer 1 bottle (100 mL)
Component D: Horseradish Peroxidase 1 vial (20 units)
Component E: DMSO 1 vial (1 mL)
Features & Benefits :
Broad Application: Can be used for quantifying HRP activities in solutions and solid surfaces (e.g, ELISA).
Sensitive: Detect as low as 3 mU/mL HRP in solution.
Continuous: Easily adapted to automation without a separation step.
Convenient: Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive: No special requirements for waste treatment.
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