Product Overview :
Creatinine assay kit is designed to measure creatinine directly in biological samples without any pretreatment. The improved Jaffe method utilizes picrate that forms a red colored complex with creatinine. The intensity of the color, measured at 510nm, is directly proportional to creatinine concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw sample.
Applications :
Direct Assays: urine, serum, plasma and biological preparations.
Drug Discovery/Pharmacology: effects of drugs on creatinine metabolism.
Storage :
The kit is shipped at room temperature. Store Reagents A and B at 2-8°C and Creatinine Standard at -20°C. Shelf life: 12 months after receipt.
Kit Components :
Reagent A: 50 mL
Reagent B: 50 mL
Creatinine Standard: 1 mL 50 mg/dL
Detection method :
OD510nm
Features & Benefits :
Sensitive and accurate. Use 30 µL samples. Detection limit 0.10 mg/dL (8µM) creatinine in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day.
Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet.
Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples.
Sensitivity :
0.1 mg/dL (8 μM)
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