Product Overview :
DUB Activity Assay Kit facilitates the rapid, robust measurement of deubiquitinating enzyme activity in vitro. The kit utilizes a high purity, fluorogenic substrate (ubiquitin-AMC) together with suitable calibration standards and controls for the accurate and sensitive assessment of DUB activity. Continuous kinetic or end-point assays can be performed in a 96-well plate format for multi-sample analysis. Each kit contains sufficient material for one full 96-well plate assay set-up to be run. Ubiquitin-AMC is not a suitable substrate for all DUBs. Compatibility must be determined by the end user.
Kit Components :
DUB Assay Buffer (10X), 1 vial/1 ml, -20°C
Control DUB enzyme (USP2 Catalytic Domain), 1 vial/25 µl, -80°C
Ubiquitin-AMC, 1 vial/25 µl, -80°C
AMC Assay Reagent, 1 vial/100 µl, -20°C
DTT (1M) Assay Reagent, 1 vial/1 ml, -20°C
96-Well Cover Sheet 1 cover RT
Half Volume 96-Well Plate (white) 1 plate RT
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