Product Overview :
E2F4 Gene Knockdown Quantification Kit is used for measuring E2F4 knockdown induced by siRNA or antisense oligonucleotide at the protein level.
Description :
Targeted gene knockdown using small interfering RNA (siRNA) or antisense oligonucleotide has been valuable technology for studying gene function. Gene knockdown leads to reduction in mRNA and subsequently protein expression. It can be often verified at mRNA level by Northern blot or quantitative RT-PCR. However, decrease in the amount of a specific mRNA does not typically correlate well with protein levels present in the cell. Gene knockdown can be also measured at the protein level with Western blot. Western blot analysis is the most comprehensive way of showing that expression of the target gene has been down regulated. However this method, while sensitive, often lacks the ability to discriminate between samples in which the differences in protein levels are minimal. It is also limited in its application to high-throughput analysis.
Applications :
E2F4 Gene Knockdown Quantification Kit For Tumor Sup-pressors/Oncogenes is suitable for quantifying gene knockdown caused by siRNA or antisense oligonucleotides using mammalian tissue and cell extracts.E2F4 Gene Knockdown Quantification Kit For Tumor Sup-pressors/Oncogenes series offers a flexible choice of different kits used for measuring knockdown of 41 common genes related to tumor Sup-pressors/oncogenes.
Usage :
For research use only (RUO)
Storage :
Upon receipt: (1) Store the Detection Antibody at –20°C; (2) Store Q2, Q4, Q6, GAPDH Control Antibody, Capture Antibody, and 88-Well Assay Strips at 4°C away from light; (3) Store all other components at room temperature. The kit is stable for up to 6 months from the shipment date, when stored properly.Note: Check if wash buffer, Q2 contains salt precipitates before using. If so, warm (at room temperature or 37°C) and shake the buffer until the salts are re-dissolved.
Kit Components :
Q1 (Extraction Bbuffer) 12 mlQ2 (10X Wash Buffer) 28 mlQ3 (Protein Capture Buffer) 1 mlQ4 (Blocking Buffer) 20 mlQ5 (Antibody Buffer) 12 mlQ6 (Developing Solution) 10 mlQ7 (Stop Solution) 6 mlGAPDH Control Antibody* 20 µlCapture Antibody* 25 µlDetection Antibody* 20 µl8-Well Assay Strips (with Frame) 12User Guide 1* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.
Detection method :
Colorimetric
Compatible Sample Types :
Cell, Tissue
Features & Benefits :
Quick and efficient. Completion of entire assay needs only 4 hours.Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.The internal control is conveniently included to correct for the variations for the cell number or protein concentrations.Strip microplate format makes the assay flexible: manual or high throughput.Simple, reliable, and consistent assay conditions.
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