Product Overview :
Free Fatty Acid Assay Kit is a quantitative colorimetric/fluorimetric fatty acid determination.
Description :
Fatty acids are aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax. Fatty Acids play important roles in cellular synthesis, energy metabolism and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome and Reye Syndrome. Free Fatty Acid Assay Kit provides a simple, one-step and high-throughput assay for measuring free fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the free fatty acid concentration in the sample.
Applications :
Assays: free fatty acids in biological samples such as serum, plasma, urine, saliva, milk, cell cultures and in food, agriculture products.Drug Discovery/Pharmacology: effects of drugs on free fatty acid metabolism.
Usage :
For research use only (RUO)
Storage :
Store all components at -20°C. Shelf life of six months after receipt.
Kit Components :
Assay Buffer 20 mLDye Reagent 120 μLEnzyme A (Dried) 1 vialEnzyme B 120 μLCoSubstrate 120 μLStandard 1 mL 1 mM palmitic acid
Detection method :
Colorimetric, Fluorometric
Compatible Sample Types :
Cell Culture, Food Sample, Milk, Plasma, Saliva, Serum, Urine
Features & Benefits :
Sensitive. Use 10 μL samples. Linear detection range: colorimetric assay 7 - 1000 μM, fluorimetric assay 7 - 100 μM fatty acid.Convenient. Room temperature " mix-and-read " procedure can be readily automated for high-throughput assay of thousands of samples per day.
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