Common Name :
Fructosamine
Product Overview :
Fructosamine Assay Kit is a microplate-based colorimetric assay for the direct determination of fructosamine levels in serum. The assay is based on the ability of fructosamine to reduce nitroblue tetrazolium (NBT), forming a colored end-product (purple) under alkaline conditions. The formation rate of formazan is proportional to the concentration of fructosamine in samples and the increase in absorbance (OD 530nm) can be monitored using a spectrophotometer. The kit includes a Thiol Blocking Reagent and a Sample Cleaning Mix that minimizes the interference of other endogenous reducing agents which ensures accurate measurements of Fructosamine in biological samples. The assay is simple, reproducible and can detect as low as 20 µmol/L of fructosamine in samples.
Applications :
Measurement of Fructosamine levels in serum
Storage :
Store the kit at -20°C. Briefly centrifuges small vials prior to opening. Read entire protocol before performing the assay.
Fructosamine Buffers A and B: Store at 4 °C. Bring to room temperature before use.
NBT: Aliquot and Store at -20°C. Protect from light. Bring to room temperature before use.
Thiol Blocking Reagent: Reconstitute with 400 µl Fructosamine Buffer A. Vortex and Mix Well. Store at -20°C. When dissolved, use within 2 month.
Sample Cleaning Mix: Reconstitute with 1 ml Fructosamine Buffer A. Allow contents to dissolve intensively. Aliquot and keep at -20°C. When dissolved, use within 2 month.
Fructosamine Calibrator: Upon received, store at 4°C. Stable for 2 months at 4 °C after opening.
Kit Components :
Fructosamine Buffer A; 25 ml
NBT (in DMF); 580 µl
Thiol Blocking Reagent; 1 vial
Sample Cleaning Mix (Lyophilized); 1 vial
Fructosamine Buffer B; 40 ml
Fructosamine Calibrator (3.2 mM); 180 µl
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