Product Overview :
Fructosamine Enzymatic Assay Kit is a plate-based colorimetric assay for the determination of Fructosamine in serum samples. The kit uses a spectrophotometric assay to detect Fructosamine directly from serum samples, enabling researchers to detect Fructosamine levels in animal serum and other tissue matrices.
Description :
Fructosamines are stable carbohydrate-protein complexes that form in the blood by glycation reactions between sugars and primary amines. In diabetes, blood sugars are measured by either monitoring current blood glucose level or by glycated hemoglobin (HbA1c). Similar to the HbA1c test, fructosamine testing calculates the fraction of total serum proteins that have undergone glycation and typically reflect albumin glycation. Since the half-life of albumin is approximately 20 days, the fructosamine level reflects 1-2 week changes in blood glucose. Therefore, fructosamine testing provides earlier and more sensitive detection for diabetes than many other carbohydrate tests.
Fructosamine Enzymatic Assay Kit is a simple, direct and automationcompatible method for measuring fructosamine levels in serum. This kit is based on the ability of ketoamines to directly reduce nitroblue tetrazolium (NBT) to colored formazan dye under alkaline conditions. The absorbance measured at 550 nm, is proportional to the concentration of fructosamine in the sample. The kit also comes with a control solution containing a fructosamine standard which can be used to calibrate the assay
Stability :
The shelf life is 6 months after receipt when the kit is properly stored. After opening, the Fructosamine Standard is stable for 30 days stored at 4°C. Reconstituted Fructosamine Reagent is stable for 30 days if stored refrigerated at 4°C.
Storage :
Store the kit at 4 °C.
Warning :
1. Add standards to plate only in the order from low concentration to high concentration, as this will minimize the risk of compromising the standard curve.
2. The assay is not influenced by hemoglobin values up to 200mg/dl or by bilirubin levels up to 20mg/dl or by glucose up to 600 mg/dl.
3. Interference from grossly hemolyzed specimens is correctable by use of a serum/plasma blank.
Kit Components :
Microtiter Plate, One, 4°C – 25 °C
Fructosamine Reagent, Bottle, 4 ºC
Reagent Buffer, 2 x 20 mL, 4 ºC
Fructosamine Standard (3.2 mmol/L), 0.6 mL, 4 ºC
Standard Dilution Buffer, 1.5 mL, 4 ºC
Features & Benefits :
1. High sensitivity and large assay range (1 - 8 mmol/L).
2. A rapid (15 minute) and robust enzyme-based assay which does not require expensive instrumentation.
3. High reproducibility.
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