Fumarate Colorimetric Assay Kit

Cat.No. :

Kit-2231

Product Overview :

Fumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb’s cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy. Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples. The fumarate Enzyme Mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (λ = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 μM).

Applications :

Kit can detect as low as 1 nmol of fumarate per well (20 μM).

Storage :

-20°C

Shipping :

Gel Pack

Size :

100 assays

Kit Components :

Fumarate Assay Buffer; Fumarate Enzyme Mix; Fumarate Developer; Fumarate Standard (0.1 M)

Target Species :

Mammalian

Detection method :

Absorbance (450 nm)

Features & Benefits :

Simple procedure; takes ~ 40 minutes; Fast and convenient