Product Overview :
GGT Activity Assay Kit (Fluorometric) is a quantitative fluorometric determination of GGT activity.
Description :
The Gamma-Glutamyl Transferase (GGT; EC 2.3.2.2) is an enzyme that transfers gamma-glutamyl functional groups. It is found in many tissues, the most notable one being the liver, and has significance in medicine as a diagnostic marker. GGT Activity Assay Kit (Fluorometric) provides a convenient tool for sensitive detection of the GGT in a variety of samples. The GGT in sample will recognize L-γ-Glutamyl-AMC as a specific substrate leading to proportional fluorescence development. The activity of GGT can be easily quantified fluorometrically (Ex/Em = 365/460 nm). This assay detects GGT activity as low as 0.02 mIU in sample.
Usage :
For research use only (RUO)
Storage :
Store the kit at -20°C, protected from light. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.
Kit Components :
GGT Assay Buffer. Cap code: WM. 25 mlGGT Substrate. Cap code: NM. 1 BottleGGT Positive Control. Cap code: green. 1 VialAMC Standard (1mM). Cap code: yellow. 100 µl
Detection method :
Fluorometric
Compatible Sample Types :
Cell Lysate, Serum, Tissue Lysate