Product Overview :
The Giardia & Cryptosporidium Water Testing Kit is designed to detect the cyst and oocyst stages of these parasites in particulates isolated from water and other environmental samples utilizing the principle of direct immunofluorescence.
» The antibody reagent consists of a mixture of fluorescein-labeled mouse monoclonal antibodies made to cyst and oocyst outer wall antigenic sites (epitopes) of Giardia lamblia and Cryptosporidium parvum. This reagent is genus-specific and will bind only to the cysts and oocysts of these two parasites if they are present. The reagent shows varying degrees of cross-reactivity with cysts and oocysts of other species of Giardia and Cryptosporidium. The cysts and/or oocysts will appear bright apple green when viewed under a fluorescence microscope using the appropriate filters for fluorescein. This antibody cross-reacts with some species of algae.
» Counterstain contains Evans Blue. It binds nonspecifically, fluorescing red using a fluorescein filter setting, enhancing contrast with the apple-green fluorescence of the specific antibody reaction.
» Mounting Medium is fade-retardant. Minimize exposure to light. Some yellowing may occur over time with exposure to light - this will not affect performance.
» DAPI (4,6-diamidino-2-phenylindole) is prepared at 2mg/mL in methanol (5000X stock solution). The volume is 0.4 mL. A 1X solution can be prepared by diluting 1 uL of DAPI in 5 mL PBS (phosphate-buffered saline solution, pH 7.4) or 10 uL diluted in 50 mL PBS. DAPI binds to DNA, fluorescing blue using a UV filter setting. Minimize exposure to light.
» Wash Buffer is a 1X working dilution buffer provided for the rinse processes. This buffer needs no dilution prior to use.
» Slides are chemically treated to increase adhesion of cysts, oocysts, spores, and other cells. The wells measure 15 mm in diameter. Each slide has a green, Tefloncoated section that is hydrophobic to contain the sample within the well. Each slide also has a frosted area at one end for writing with pencil or marker. Packaged forty slides per box.
» Positive Control is a mixture of Giardia lamblia cysts and Cryptosporidium parvum oocysts in a mixed aldehyde buffer. The concentration of this suspension is approximately 2x10e5 cysts and oocysts (each) per mL. (These numbers are not exact and should not be used for sample recovery estimation.)
Description :
Giardia lamblia and Cryptosporidium parvum are common, ubiquitous intestinal parasitic protozoa that cause gastroenteritis in man and lower animals. Both organisms have a reservoir of host animals and can be spread through fecal contamination of food, water, and fomites. The Cryptosporidium oocyst is a nearly round encysted organism of approximately 3-5 um in diameter, while the Giardia cyst is oval-shaped and measures approximately 8-13 um in length and 7-10 um in width.
Storage :
Store at 4° C. DO NOT FREEZE.
A100FLR-1X reagent, C101, D101, and M101 are light sensitive.
Kit Components :
• A100FLR-1X: 1 dropper vial G/C antibody containing 3.5 mL working dilution (1x) reagent
• PC101: 1 glass vial containing 1 mL positive control
• WB101: 1 screw cap bottle containing 50 mL 1x Wash Buffer
• C101: 1 dropper vial containing 3.5 mL counterstain
• D101: 1 microtube containing 0.4 mL DAPI, 5000X in methanol
• M101: 1 dropper vial containing 3.5 mL Mounting Medium
• S100-2: 1 box of two-well Slides, 40/box
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