Product Overview :
Glutamate Dehydrogenase Detection Kit provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.
Description :
Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to α-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.
Applications :
Functional Studies
Storage :
Store kit at -20°C, protect from light. Warm the Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening. Read the entire protocol before performing the assay.
Kit Components :
Components Identifier 100 testsGDH Assay Buffer WM 1 x 25mlGDH Developer Red 1 vialGDH Positive Control Green 1 vialGlutamate Blue 1 x 1mlNADH Yellow 1 vial
Compatible Sample Types :
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Sensitivity :
>0.01 mU/well
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