Glutathione Reductase Assay Kit

Cat.No. :

Kit-0391

Product Overview :

Glutathione Reductase Assay Kit is used for measuring GR activity.

Description :

Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. The Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with5, 5"-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.

Target Species :

Mammals

Usage :

For research use only (RUO)

Storage :

Store kit at -20°C, protect from light. Warm Assay Buffer to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.

Kit Components :

GR Assay Buffer 100 ml3% H2O2 1 mlCatalase (lyophilized) 1 vialTNB Standard (lyophilized) 1 vialDTNB (lyophilized) 1 vialNADPH-GNERAT (lyophilized) 2 vialsGSSG (lyophilized) 1 vialGR Positive Control (10 mU; lyophilized) 1 vial

Detection method :

Colorimetric

Compatible Sample Types :

Biological Fluid, Cell Culture Supernatant, Erythrocyte Lysate, Plasma, Serum, Tissue Culture Supernatant, Urine