Product Overview :
Hemoglobin Assay Kit is a colorimetric determination of total hemoglobin at 400 nm.
Description :
Hemoglobin (Hb) is made of four globin chains each carrying a heme group. It is carried by red blood cells and transports oxygen from the lungs to the peripheral tissues to maintain the viability of cells. Quantitation of blood hemoglobin has been a key diagnostic parameter for various diseases such as anemia, polycythemia and dehydration.Simple, direct and automation-ready procedures for measuring hemoglobin concentration are becoming popular in Research and Drug Discovery. Hemoglobin Assay Kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 400 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation exhibits high sensitivity and is ideal for measureing hemolysis in low hemoglobin samples (e.g. serum and plasma).
Applications :
Direct Assays: total hemoglobin in serum, plasma, urine, etc.Pharmacology: effects of drugs on hemoglobin metabolism.Drug Discovery: HTS for drugs that modulate hemoglobin levels.
Usage :
For research use only (RUO)
Storage :
Store reagent and Calibrator at 4°C. Shelf life: 12 months after receipt.
Kit Components :
HB Reagent 50 mLCalibrator 10 mL
Detection method :
Colorimetric
Compatible Sample Types :
Blood, Plasma, Serum, Urine
Features & Benefits :
Sensitive and accurate: Linear detection range 0.9 – 200 mg /dL hemoglobin in 96-well plate assay.Simple and high-throughput: The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.Safety: Reagents are non-toxic.Versatility: Assays can be executed in 96-well plate or cuvette.
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