- Quantification of trypsin activity in cell culture supernatants and biological fluids - Enzyme kinetic studies and inhibitor screening in research settings - Monitoring of cell dissociation and subculture processes - Assessment of trypsin activity in enzyme preparations and formulations
Product Overview :
This kit measures trypsin activity in biological samples using a fluorogenic substrate. Compared to traditional BAEE (Nα-Benzoyl-L-arginine ethyl ester) or TAME (p-Toluenesulfonyl-L-arginine methyl ester) activity assays, this fluorometric format delivers significantly higher sensitivity for detecting low-level trypsin activity.
Features & Benefits :
- Fluorometric detection provides higher sensitivity than conventional BAEE/TAME colorimetric methods - Enables accurate measurement of low trypsin activity in dilute or low-concentration samples - Compatible with standard fluorescence microplate readers (Ex/Em ~360/460 nm) - Homogeneous assay format eliminates the need for separation or wash steps