His-tagged Protein Purification Kit

Common Name :

Protein

Cat.No. :

Kit-0887

Product Overview :

Ni-NTA Magnetic nano beads are silica beads, with an average diameter of 0.4 μm and a range of 0.2~0.8 μm diameter, that contain magnetic particles and have strongly metal-chelating nitrilotriacetic acid (NTA) groups covalently bound to their surfaces.
They are precharged with nickel and ready to use for capturing 6xHis-tagged proteins under native conditions for protein expression screening programs, as well as smallscale purification of 6xHis-tagged proteins.
Ni-NTA Magnetic nano Beads are supplied as a 10% (v/v) suspension with a binding capacity of 3 mg protein per ml of suspension for 6xHis-tagged DNA polymerase

Storage :

Ni-NTA Magnetic nano beads are supplied as a 10% (v/v) suspension in 20% ethanol and should be store at 2~8°C.

Kit Components :

Ni-NTA magnetic Nano Beads 5 X 1 mL(10%);
Binding/Washing buffer 100 mL;
Elution buffer 15 mL;
Nd magnet 3 ea;
User’s Guide 1 ea;
Manual 1 ea.

Features & Benefits :

Flexible: The protocol may be carried out with a magnet or via centrifugation.
Excellent performance: Highly pure proteins are obtained through our exclusive SSMB (Spherical-Shape Magnetic Beads).
High purity and High yield: Average binding capacity of 500 μg protein per ml of suspension for 6xHis-tagged protein. Purity that is > 90%.
Reproducibility: The only hands-on step is the addition of protein extract, maximizing reproducibility.
Fast process: The entire purification is complete in about 30 minutes!