Product Overview :
A selective indicator for the detection of Highly Reactive Oxygen Species, hROS.
Description :
A new novel probe, Aminophenyl fluorescein (APF), developed by Tetsuo Nagano et. al. (1), is a general selective indcator for the detection of highly Reactive Oxygen Species (hROS). The probe has little reactivity towards other ROS such as: singlet oxygen (O21), superoxide (O2-•), hydrogen peroxide (H2O2), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1. APF is a cell permeable indicator that can be used to detect Hydroxyl Radical (.OH), Peroxynitrite: (ONOO-) and hypochlorite (-OCl) production in cells.Several fluorescent probes such as 2",7"-dichlorodihydrofluorescein (DCFH) and di-hydrorhodamine123, have been developed to detect Reactive Oxygen Species (ROS). As Hempel and co-workers have indicated, DCFH and di-hydrorhodamine123 can react with various ROS and oxidizing species such as hydrogen peroxide, hypochlorite, superoxide, nitric oxide, ferrous ion, and others. These probes should be considered as detecting a broad range of ROS.
Applications :
Flow Cytometer, Fluorescence Microscope, Fluorescence plate reader
Usage :
1. For Research use only. Not for use in diagnostic procedures.2. Practice safe laboratory procedures by wearing protective clothing and eyewear.
Storage :
1. Long Term Storage: Store contents as labeled.2. Upon Arrival: 2-8°C.3. Aminophenyl fluorescein (APF) and Hydroxyphenyl fluorescein (HPF) should be stored at 4-80C. Protect from light until ready to use. The diluted material must be used immediately and discard any unused diluted material.
Kit Components :
One vial of APF
Features & Benefits :
1. Quenched Cell permeable dye.2. One Step, No wash Homogenous assay.3. Adaptable to High throughput assay platforms.4. Can monitor multiple time points to follow real time kinetics.5. Non-destructive cell based assay allows monitoring of additional parameters.6. Applications-Fluorescent plate reader/Flow Cytometery/Fluorescent Microscopy.
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