Description :
Hydrogen Peroxide is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-κB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases. Hydrogen Peroxide Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples. In the presence of Horse Radish Peroxidase (HRP), the OxiRed Probe reacts with H2O2 to produce product with color (λmax = 570 nm) and red-fluorescent (Ex/Em=535/587 nm). The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H2O2 in the sensitive fluorometric assay.
Applications :
The kit provides a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring H2O2 in biological samples.
Kit Components :
H2O2 Assay Buffer; OxiRed; Probe; Dimethylsulfoxide (DMSO, anhydrous); HRP; H2O2 Standard (0.88M)
Target Species :
Mammalian
Detection method :
Absorbance (570 nm)and fluorescence(Ex/Em 535/587 nm)
Features & Benefits :
Simple procedure; takes less than ~1 hours;
Fast and convenient;
The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method.
The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H2O2 in the sensitive fluorometric assay.
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