Product Overview :
Hydroxyphenyl fluorescein (HPF) is a highly selective, cell permeable fluorescent probe for the detection of highly Reactive Oxygen Species (hROS): hydroxyl radical (OH•), and peroxynitrite (ONOO-) detection. It has little reactivity towards other hROS such as: hypochlorite (-OCl), singlet oxygen (O2), superoxide (O2-•), hydrogen peroxide (H2O2), nitric oxide (NO•), and alkyl peroxide (RO2•).
Description :
A new novel probe, Hydroxyphenyl fluorescein (HPF), developed by Tetsuo Nagano et. al. (1), is a highly selective probe for the detection of highly Reactive Oxygen Species (hROS). It is a cell permeable highly sensitive fluorescent probe for hydroxyl radical (OH•), and peroxynitrite (ONOO-) detection. It has little reactivity towards other hROS such as: hypochlorite (-OCl), singlet oxygen (021), superoxide (02-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•).
Applications :
Fluorescence plate reader
Usage :
1. For Research use only. Not for use in diagnostic procedures.2. Practice safe laboratory procedures by wearing protective clothing and eyewear.
Storage :
1. Long Term Storage: Store contents as labeled.2. Upon Arrival: 2-8°C.3. Aminophenyl fluorescein (APF) and Hydroxyphenyl fluorescein (HPF) should be stored at 4-80C. Protect from light until ready to use. The diluted material must be used immediately and discard any unused diluted material.
Kit Components :
1. Kit:Hydroxylradical/Peroxynitrite Detection1) 1 vial: HPF 5mM solution in DMF2. Kit:hROSDetection1) 1 vial: APF 5mM solution in DMF3. Kit:HypochloriteDetection1) 1 vial: APF: 5mM solution in DMF.2) 1 vial: HPF: 5mM solution in DMF.
Features & Benefits :
1. Quenched Cell permeable dye.2. One Step, No wash Homogenous assay.3. Adaptable to High throughput assay platforms.4. Can monitor multiple time points to follow real time kinetics.5. Non-destructive cell based assay allows monitoring of additional parameters.6. Applications-Fluorescent plate reader/Flow Cytometery/Fluorescent Microscopy.
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