Product Overview :
This Cell Intracellular Fluorimetric Hydrogen Peroxide Assay Kit uses our unique Green Peroxide Sensor to quantify hydrogen peroxide in live cells. Green peroxide sensor is cellpermeable, and generates the green fluorescence when it reacts with hydrogen peroxide. This kit provides a sensitive tool to monitor hydrogen peroxide level in living cells. The kit is also optimized with "mix and read" assay format for solution based assay. It provides a sensitive, one-step fluorimetric assay to detect as little as 0.3 nanomoles of H2O2 in a 100 µL assay volume (3 µM). The assay can be performed in a convenient 96-well or 384-well microtiterplate format. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 490/520 nm for H2O2 detection in solution or a fluorescence microscopy and a flow cytometry for live cell H2O2 detection.
Description :
Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in many biological events that are linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. The measurement of this reactive species is helpful for determining how oxidative stress modulates various intracellular pathways.
Storage :
Keep in freezer and avoid exposure to light.
Kit Components :
Component A: Green Peroxide Sensor 1 vial
Component B: H2O2 1 vial (3% stabilized solution, 200 μL)
Component C: Assay Buffer 1 bottle (20 mL)
Component D: DMSO 1 vial (200 μL)
Features & Benefits :
Broad Application: Can be used for quantifying hydrogen peroxide in live cells, in solutions, and in cell extracts.
Continuous: Easily adapted to automation without a separation step.
Convenient: Formulated to have minimal hands-on time. No wash is required.
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