Product Overview :
Intracellular Total ROS Activity Assay Kit (Green Fluorescence) is used for the measurement of intracellular total ROS activity.
Description :
Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways. Intracellular Total ROS Activity Assay Kit (Green Fluorescence) uses our unique ROS sensor to quantify ROS in live cells. ROS Green is cell-permeable. It generates the green fluorescence when it reacts with ROS. The kit is an optimized "mix and read" assay format that is compatible with HTS liquid handling instruments.
Applications :
Detection of ROS in Jurkat cells.
Usage :
For research use only (RUO)
Storage :
Keep in freezerAvoid exposure to lightAvoid repeated freeze-thaw cycles
Kit Components :
Component A: ROS Green 1 vialComponent B: Assay Buffer 20 mLComponent C: DMSO 200 µL
Detection method :
Fluorometric
Compatible Sample Types :
Live Cells
Features & Benefits :
Broad Application: Can be used for quantifying ROS in live cells.Continuous: Easily adapted to automation without a separation step.Convenient: Formulated to have minimal hands-on time.Non-Radioactive: No special requirements for waste treatment.
PDF