Product Overview :
The LCAT Activity Assay Kit is a fluorometric assay useful for measuring the phospholipase activity of LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman’s reagent) LCAT inhibitor. Applications for this method include high-throughput screening, mechanism of action studies, and structure-activity relationship (SAR) work.
Storage :
The kit is shipped on wet ice. Storage at -20 °C, protected from light, is recommended. Components are stable for 1 year, if stored properly.
Handling :
Briefly centrifuge vials before opening.
Kit Components :
Substrate Reagent: 100 µl, -20°C
READ Reagent: 30 ml, 4°C
Assay Buffer: 20 ml, 4°C
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