Description :
Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help diagnose Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5 - 10 fold increase of serum lipase levels within 24 to 48 hours. In the Lipase Assay Kit, lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by via monitoring a linked change in the OxiRed probe absorbance (λ = 570nm). This assay is rapid, simple, sensitive, and reliable, as well as, suitable for high throughput activity screening of lipase.
Storage :
Store the kit at -20°C, protect from light. Allow Assay Buffer to warm to room temperature before use. Briefly centrifuge vials before opening.
Kit Components :
Assay Buffer, 1 x 25ml
Enzyme Mix (lyophilized), 1 vial
Glycerol Standard, 1 x 0.2ml
Lipase positive control, 1 vial
Lipase Substrate, 1 vial
OxiRed Probe, 1 x 0.2ml
Target Species :
Mammalian
Detection method :
Absorbance (570 nm)
Features & Benefits :
• Simple procedure; takes ~ 60-90 minutes
• Fast and convenient
• Sensitive assays for measuring Lipase activity in various biological samples.
Sensitivity :
0.02 mU/well
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