Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit

Common Name :

MDA

Cat.No. :

Kit-0542

Product Overview :

Simple procedure
Fast and convenient
Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples

Description :

Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. Lipid Peroxidation Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (λ = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

Applications :

The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

Usage :

For Research Use Only! Not For Use in Humans.

Storage :

-20°C

Size :

100 assays

Kit Components :

• MDA Lysis Buffer
• Phosphotungstic Acid Solution
• BHT (100X)
• TBA
• MDA Standard (4.17M)

Target Species :

All

Detection method :

Absorbance (532 nm) or Fluorescence (Ex/Em 532/553 nm)

Compatible Sample Types :

Cell and Tissue culture supernatants, plasma and other biological fluids (optimized by end user).