Lipoxygenase Inhibitor Screening Assay Kit detects and measures the hydroperoxides produced in the lipoxygenation reaction using a purified Lipoxygenases (LOs). The detection reaction is equally sensitive to hydroperoxides at various positions within the fatty acid, and will work with fatty acids of any carbon length. It is thus a general detection method for LO, and can be used to screen libraries of compounds for those which inhibit LO enzymes.
Applications :
Functional Studies
Storage :
Kit will arrive packaged as a -20°C kit. For best results, remove components and store as stated below Item Storage Lipoxygenase Inhibitor Screening Assay Buffer (10X) 4°C Developing Reagent 1 4°C Developing Reagent 2 4°C 15-Lipoxygenase Standard 4°C Arachidonic Acid (substrate) -20°C Linoleic Acid (substrate) -20°C Potassium Hydroxide 4°C Nordihydroguaiaretic Acid (NDGA) Positive Control Inhibitor -20°C 96-Well Solid Plate (Colorimetric Assay) RT 96-Well Cover Sheet RT
Under the standard conditions described in this booklet, samples containing LO activity between 1-10 nmol/min/ml can be assayed without further dilution or concentration. The assay will detect 0.5-5 nmol of lipid hydroperoxides.
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