Product Overview :
Lipoxygenase Inhibitor Screening Assay Kit detects and measures the hydroperoxides produced in the lipoxygenation reaction using a purified Lipoxygenases (LOs). The detection reaction is equally sensitive to hydroperoxides at various positions within the fatty acid, and will work with fatty acids of any carbon length. It is thus a general detection method for LO, and can be used to screen libraries of compounds for those which inhibit LO enzymes.
Applications :
Functional Studies
Storage :
Kit will arrive packaged as a -20°C kit. For best results, remove components and store as stated below
Item Storage
Lipoxygenase Inhibitor Screening Assay Buffer (10X) 4°C
Developing Reagent 1 4°C
Developing Reagent 2 4°C
15-Lipoxygenase Standard 4°C
Arachidonic Acid (substrate) -20°C
Linoleic Acid (substrate) -20°C
Potassium Hydroxide 4°C
Nordihydroguaiaretic Acid (NDGA) Positive Control Inhibitor -20°C
96-Well Solid Plate (Colorimetric Assay) RT
96-Well Cover Sheet RT
Kit Components :
Components 96 tests
15-Lipoxygenase Standard 1 vial
96-Well Plate (Colorimetric Assay) 1 plate
96-Well Plate Cover 1 cover
Arachidonic Acid (substrate) 1 vial
Developing Reagent 1 1 vial
Developing Reagent 2 1 vial
Linoleic Acid (substrate) 1 vial
Lipoxygenase Inhibitor Screening Assay Buffer (10X) 1 vial
Potassium Hydroxide 1 vial
Nordihydroguaiaretic Acid (NDGA) Positive Control Inhibitor 1 vial
Sensitivity :
Under the standard conditions described in this booklet, samples containing LO activity between 1-10 nmol/min/ml can be assayed without further dilution or concentration. The assay will detect 0.5-5 nmol of lipid hydroperoxides.
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