Product Overview :
The Live/Dead Assay Kit provides a two-color fluorescence assay that detects both live and dead cells for mammalian cell types.
Description :
This kit enables the detection of both the live and dead populations of mammalian cells based on the integrity of the cell membrane using a fluorescence microplate reader. Calcein AM is a widely used live-cell marker. The non-fluorescent calcein AM permeates the intact cell membrane and is converted into the fluorescent calcein by intracellular esterases. The number of live cells is therefore indicated by the intensity of green fluorescence in the cytosol, with excitation at 495 nm and emission at 530 nm. EthD-III is virtually non-fluorescent and impermeant to an intact plasma membrane. In the event of compromised cell membrane integrity that is associated with cell death, EthD-III enters cells and binds to nucleic acids, resulting in bright red fluorescence in dead cells, with excitation at 530 nm and emission at 645 nm.
Stability :
When stored as directed, the kit is stable for at least 6 months from the date it is received.
Storage :
Reagents in this kit should be stored sealed, desiccated, protected from light, and frozen at -20°C. Allow the reagents to warm up to room temperature before opening. Before refreezing, seal all stock solutions tightly. Calcein AM is susceptible to hydrolysis when exposed to moisture. If the color of Calcein AM stock solution turns orange, discard the tube. EthD-III is stable and insensitive to moisture. Stock solutions of EthD-III in DMSO/H2O or other aqueous media can be stored frozen at -20°C.
Synonyms :
Cell Assay Kit
Kit Components :
Calcein AM (4 mM, 30 µL)
EthD-III (2 mM, 60 µL)
Each kit contains enough material for two (2) 96-well or 384-well microplates.
Features & Benefits :
Optimized for fluorescence microplate readers, such as SpectraMax® readers
Simple workflow-direct measurement in wells with or without medium removal
Increased sample throughput with microplate format
Preconfigured protocol in SoftMax® Pro Software
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