Product Overview :
Lactate Dehydrogenase Assay Kits provide both fluorescence and absorbance-based method for detecting either L-LDH or D-LDH in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, LDH is proportionally related to the concentration of NADH that is specifically monitored by a fluorogenic NADH sensor. This assay kit is specific for L-LDH. The signal can easily read by an absorbance microplate reader at ~575 nm or at the absorbance ratio of A575nm/A605nm to increase assay sensitivity. With the L-Lactate Dehydrogenase Assay Kit, we were able to detect as little as 3mU/mL LLDH in a 100 µL reaction volume.
Description :
The Malachite Green Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be executed in tubes, cuvettes or multi-well plates. The assays can be conveniently performed in 96- and 384-well plates for high-throughput screening of enzyme inhibitors.
Applications :
Phosphatase Assays: liberation of phosphate from peptide, protein or small molecule substrate.
Lipase Assays: liberation of phosphate from phospholipids
Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc).
Quantitation of Phosphate in phospholipids, proteins and DNAs, etc.
Drug Discovery: high-throughput screen for phosphatase inhibitors.
Storage :
The kit is shipped at ambient temperature. The reagents and standard are stable for one year when stored at 4°C.
Kit Components :
Reagent A: 50 mL
Reagent B: 1 mL
Standard: 1mL 1 mM phosphate
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