Product Overview :
MDH Activity Assay Kit (Colorimetric) is used for measuring malate dehydrogenase activity using colorimetric methods.
Description :
Malate Dehydrogenase (MDH) (EC 1.1.1.37) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic & participates in the malate- aspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer's disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma). In Malate Dehydrogenase Activity Assay kit, MDH reacts with malate to form an intermediate. The generated intermediate reacts with MDH Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect less than 0.5 mU of MDH activity in various sample types.
Applications :
Measurement of malate dehydrogenase activity in various tissues/cells
Analysis of citric acid cycle and malate-asparate shuttle
Usage :
For research use only (RUO)
Storage :
Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read the entire protocol before performing the experiment.
Kit Components :
MDH Assay Buffer 20 ml
MDH Substrate (Lyophilized) 1 vial
MDH Enzyme Mix (Lyophilized) 1 vial
MDH Developer (Lyophilized) 1 vial
NADH Standard (Lyophilized) 1 vial
MDH Positive Control (Lyophilized) 1 vial
Detection method :
Colorimetric
Compatible Sample Types :
Cell culture, Tissue samples
PDF