Micro NO Content Assay Kit

Cat.No. :

Kit-3734

Product Overview :

Significance: Nitric oxide (NO) is widely distributed in the nervous, cardiovascular, respiratory, digestive, and urogenital systems, with particularly high abundance in neural tissues. As a signaling molecule, NO plays important roles in physiological and pathological processes.
Principle: NO is readily oxidized to nitrite (NO₂^-) in vivo or in aqueous solutions. Under acidic conditions, nitrite reacts with sulfanilamide to form a diazonium compound, which subsequently couples with N-(1-naphthyl)ethylenediamine to produce a chromophore exhibiting a characteristic absorbance at 550 nm. Sample blanks are required to eliminate interference from endogenous pigments.

Size :

100t/96s

Storage :

Store at -20 °C. Shelf life: 6 months.

Detection method :

Spectrophotometer/Microplate Reader