Mitochondrial Complex I/NADH-CoQ Reductase Activity Assay Kit

Cat.No. :

Kit-3868

Product Overview :

Significance: Mitochondrial Complex I (EC 1.6.5.3), also known as NADH–CoQ reductase or NADH dehydrogenase, is the largest protein complex in the mitochondrial inner membrane. It catalyzes electron transfer from NADH to coenzyme Q and is a major site of superoxide generation in the respiratory electron transport chain. Its activity reflects both ETC function and reactive oxygen species production.
Principle: Complex I catalyzes the oxidation of NADH to NAD⁺. The rate of NADH oxidation is determined by monitoring the decrease in absorbance at 340 nm.

Size :

100t/96s

Storage :

Store at -20 °C. Shelf life: 6 months.

Detection method :

Spectrophotometer/Microplate Reader