Product Overview :
Mitochondrial complex V, also known as F1F0-ATP synthase, is widely distributed in mitochondria of animals, plants, microorganisms, and cultured cells. It consists of F1 and F0 subunits and utilizes the proton electrochemical gradient generated by the respiratory chain to synthesize ATP, while also catalyzing ATP hydrolysis in the reverse reaction. Complex V is also present in chloroplasts, heterotrophic bacteria, and photosynthetic bacteria and is a key enzyme in oxidative phosphorylation and photophosphorylation.
Complex V activity is determined by measuring the rate of inorganic phosphate (Pi) generation during ATP hydrolysis.