Product Overview :
This kit is designed to monitor the activity of an MMP-3 enzyme. The poptide sequence used in the kit is more selective for MMP-3 hydrolysis than other MMP enzymes. It can also be used to screen MMP-3 inhibitors when a purified MMP-3 enzyme is used. With excellent fluorescence quantum yield and longer wavelength, TF2 shows less interference from autofluorescence of test compounds and cellular components and is much more sensitive than an EDANS/Dabcyl FRET substrate. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/525 nm. The perfect excitation of TF2 at 488 nm makes the assay readily readable with almost all the common fluorescence instruments equipped with Argon laser and FITC filter set.
Description :
MMP-3 (Matrix Metalloproteinase-3), also known as stromelysin-1, is involved in wound repair, atherosclerosis and tumors. Our Fluorimetric MMP-3 Activity Assay Kit uses a Tide Fluor 2 (TF2)/Tide Quencher 2 (TQ2) fluorescence resonance energy transfer (FRET) peptide as the MMP-3 activity indicator. In the intact FRET peptide, the fluorescence of TF2 is quenched by TQ2. Upon cleavage into two separate fragments by MMP-3, the green fluorescence of TF2 is recovered.
Storage :
Store at -20 °C and keep from light. Component C can be stored at 4 °C for convenience
Kit Components :
Component A: MMP-3 Green Substrate 1 vial (60 uL), protected from light
Component B: APMA, 4-Aminophenylmercuric Acetate 1 vial (20 uL, 1 M)
Component C: Assay Buffer 1 bottle (20 mL)
Features & Benefits :
Optimized Performance: Optimized conditions for the detection of MMP-3 protease activity.
Continuous: Easily adapted to automation without a separation step.
Convenient: Formulated to have minimal hands-on time. No wash is required.
Non-Radioactive: No special requirements for waste treatment.
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