Product Overview :
Nitrate/Nitrite Fluorometric Assay Kit is a measurement for detecting total nitratr/nitrite concentration.
Description :
Nitric Oxide (NO) is synthesized in biological systems by the enzyme Nitric Oxide Synthase (NOS). NOS is a remarkably complex enzyme which acts on molecular oxygen, arginine, and NADPH to produce NO, citrulline, and NADP+. This process requires five additional cofactors (FMN, FAD, Heme, calmodulin, and tetrahydrobiopterin) and two divalent cations (calcium and heme iron). Three distinct isoforms of NOS have been identified.
Usage :
For research use only (RUO)
Storage :
The kit will perform as specified if components are stored as directed below and used before the expiration date indicated on the outside of the box.Item StorageNitrate/Nitrite Assay Buffer 4°CNitrate Reductase Enzyme Preparation -20°CNitrate Reductase Cofactor Preparation -20°CNitrate Standard 4°C or Room TemperatureNitrite Standard 4°C or Room TemperatureDAN Reagent 4°CSodium Hydroxide (2.8 M) 4°C or Room Temperature96-Well Plate (white) Room Temperature96-Well Cover Sheet Room Temperature
Kit Components :
Nitrate/Nitrite Assay Buffer 1 vialNitrate Reductase Enzyme Preparation 2 vialsNitrate Reductase Cofactor Preparation 2 vialsNitrate Standard 1 vialNitrite Standard 1 vialDAN Reagent 1 vialSodium Hydroxide (2.8 M) 1 vial96-Well Plate (white) 3 plates96-Well Cover Sheet 3 covers
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