Product Overview :
NAD (P)H dehydrogenase [quinone] 1 (NQO1) Activity Assay kit is designed for the sensitive and accurate measurement of NQO1 activity in a number of sample types.NQO1 serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways, NQO1 protects against quinone-induced damage by competing with potentially toxic one-electron pathways. It also functions in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.The enzyme activity is determined by following the reduction of Menadione with cofactor NADH and the simultaneous reduction of WST1 which leads to increased absorbance at 440 nm.
Description :
Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR" + electron donor (2 e-) + 2H+ → ROH + R"OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.
Storage :
Store kit at 4ºC immediately upon receipt.
Kit Components :
Components 1 x 96 tests
1000X Cofactor 1 vial
1000X Inhibitor 1 vial
100X Dye (lyophilized) 1 vial
100X Menadione 1 vial
100X NADH 1 vial
20X Basic Buffer 1 x 3ml
2X Extraction Buffer 1 x 15ml
96-well microplate 2 x 96-Wells
Target Species :
Mouse, Rat, Cow, Human
Compatible Sample Types :
Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts, Cell Lysate, Tissue Homogenate, Tissue Lysate
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