Product Overview :
Kit-1108 is a patented double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. Kit-1108 consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively).
Description :
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Applications :
• Kits ensure dose-dependent exosome detection in a variety of samples. No initial exosome purification required. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.
• Exosome comprehensive profiling.
• Pre-clinical researchon non-invasive biomarkers for detection and monitoring of anumber of pathologicalconditions (inflammation, cancer, neurodegeneration, etc). User friendly and suitable for multiple marker analyses.
• Up to 100 µl of biological sample can be loaded per well. Concentrated cell culture supernatant samples are recommended prior capture according to our suggested protocol.
Storage :
All the reagents are shipped and stored at 4°C for up to 24 months, if unopened. Briefly centrifuge small vials prior to opening. DO NOT FREEZE!
Kit Components :
Sample Buffer (1X): Buffer for antibody dilution and incubation, 2 bottles (2 x 10 ml)
Washing Buffer (25X): Buffer for washing plate, 1 bottle (25 ml)
Primary Antibody: α-CD9 mouse antibody, 1 vial (20 μl)
HRP Conjugated: Streptavidin HRP-conjugated secondary antibody, 1 vial (5 μl)
Substrate Chromogenic Solutions: Substrate for chromogenic detection, 1 bottle (10 ml)
Stop Solution: Stop solution, 1 bottle (10 ml)
Exosome Standards: Lyophilized exosomes from COLO-1 cell culture supernatant, 2 vials (2 x 100 μg)
Immunoplate (Transparent): Immunoplate standard multiwell plates 96-well format where assays can be conducted as singletons and/or multiple wells, 1 plate
Compatible Sample Types :
Cell culture supernatant
Sensitivity :
The sensitivity of the kit was compared to Western blot. The data reported demonstrate that the sensitivity of the kit is higher than that of Western blotting.
PDF