Description :
3-Phosphoadenosine-5-phosphosulfate (PAPS) is the universal sulfur donor for sulfotransferases. It is synthesized from ATP through two steps. In the first step, one ATP molecule is converted to adenosine-5-phosphosulfate (APS) by ATP sulfurylase and pyrophosphate (PPi) is generated as a byproduct. In the second step, the APS is converted to PAPS by APS kinase and ADP is generated as a byproduct. To drive the reaction forward, the pyrophosphate can be further degraded to phosphate (Pi) by inorganic pyrophosphatase to eliminate feedback inhibition, and the ADP can be converted back to ATP using pyruvate kinase.
Storage :
Upon receipt, store all kit components at -20 °C.
Synonyms :
3-Phosphoadenosine-5-phosphosulfate; PAPS; 3-Phosphoadenosine-5-phosphosulfate Synthesis Kit
Kit Components :
PAPS Enzyme Mix (1 vial) - 25 uL in Tris buffer at pH 7.5 with 25% glycerol. The mix contains ATP sulfurylase, APS kinase, inorganic pyrophosphatase, and pyruvate kinase at the appropriate concentrations for PAPS synthesis.
PAPS Substrate Mix (1 vial) - 100 uL containing sufficient concentrations of ATP, phosphoenolpyruvate, and enzyme co-factors.
6X Gel Loading Dye (1 vial) - 1 mL in 20 mM Tris (pH 8.0) with 30% glycerol and 0.02% bromophenol blue.
PAPS Storage Buffer (1 vial) - 1 mL of a 10X solution of 0.1 M Tris, pH 8.0. Prior to use, dilute to a 1X solution using deionized or distilled water.
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