PDE Activity Colorimetric Assay Kit

Cat.No. :

Kit-0668

Product Overview :

PDE Activity Assay Kit (Colorimetric) combines a special dual enzyme system with Green Assay Reagent for phosphate detection to create a unique, non-radioactive, colorimetric assay to detect phosphodiesterase (PDE) activity. This HTS-friendly, mix and read system may be used to screen inhibitors and modulators of cyclic nucleotide phosphodiesterase activity. 96-well microplate format permits rapid assays of large numbers of samples. The basis for the assay is the cleavage of cAMP or cGMP by a cyclic nucleotide phosphodiesterase. The kit includes a Type I PDE as positive control and a non-specific PDE inhibitor, 2-isobutyl-1-methylzanthine (IBMX) as test control for inhibitor screening.

Storage :

-80°C

Shipping :

Dry Ice

Size :

96 tests

Kit Components :

3',5'-cAMP Substrate: 1 x 2ml;
3',5'-cGMP Substrate: 1 x 2ml;
5'-AMP standard: 1 x 1ml;
5'-GMP Standard: 1 x 1ml;
5'-Nucleotidase (from Crotalus atrox venom): 1 x 1ml;
96-well Clear Microplate (1/2 Volume): 1 unit;
Desalting Column: 1 unit;
Desalting Resin: 1 x 1g;
Green Assay Reagent: 1 x 20ml;
PDE Assay Buffer: 1 x 40ml;
PDE Enzyme (from bovine brain): 5 vials*4U;
IBMX (PDE Inhibitor): 1 x 200µl