Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric) measures the activity of PAL via a two-step plate-based assay. The products of the PAL enzymatic reaction, generated in the first step, react with the developers resulting in the formation of a fluorescent compound, which is detected at Ex/Em = 410/470 nm. The kit can detect as low as 3 μU (3 pmol substrate catalysis/minute) of PAL under assay conditions.
Storage :
On receipt entire assay kit should be stored at -20°C, protected from light. Kit has a storage time of 1 year from receipt, providing components have not been reconstituted.
Shipping :
On ice
Kit Components :
Ammonium Standard II 1 x 100μl -20°C; Detection Reagent 1 x 1.2ml -20°C; Microplate Sealing Film 1 unit -20°C; PAL Assay Buffer 1 x 40ml -20°C; PAL Positive Control 1 vial -20°C; PAL Substrate 1 vial -20°C;