Phosphoglycerate Kinase Activity Assay Kit (Colorimetric)

Short Name :

Phosphoglycerate Kinase Activity Assay Kit (Colorimetric)

Cat.No. :

KITZ-009

Size :

100 tests

Product Overview :

Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) provides a quick and easy way for monitoring PGK activity in various samples. In the first step of this enzymatic assay, PGK converts 3-Phosphoglycerate and ATP to 1,3-Bisphosphoglycerate and ADP. The nascent intermediate is detected via a series of enzymatic reactions that lead the oxidation of NADH to NAD, which can be easily detected (OD= 340 nm). The assay is simple, sensitive and can detect Phosphoglycerate Kinase Activity lower than 50mU in variety of samples.

Storage :

Store kit at -20°C in the dark immediately on receipt and check below for storage for individual components. Kit can be stored for 1 year from receipt, if components have not been reconstituted. Avoid repeated freeze-thaws of reagents.

Shipping :

Gel pack

Kit Components :

PGK Assay Buffer: 25 mL -20°C
PGK Substrate: 1 vial -20°C
ATP: 2 vials -20°C
PGK Developer: 1 vial -20°C
NADH: 1 vial -20°C
PGK Positive Control: 1 vial -20°C
U.V. Transparent Plate (96-well): 1 unit -20°C

Detection method :

Colorimetric, OD = 340 nm