Product Overview :
Plant Chromatin Extraction Kit is used for isolating chromatin or DNA-protein complex from plants.
Description :
Chromatin immunoprecipitaton (ChIP) offers an advantageous tool for studying protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the specific sequences of a gene in living cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) techniques. When performing ChIP, chromatin or DNA-protein complex in plant tissues need to be first isolated in an efficient manner. However, the existing methods used for plant chromatin preparation are inconvenient and time consuming.
Applications :
The Plant Chromatin Extraction Kit is suitable for isolating chromatin or DNA-protein complex from plants in a simple and rapid format. Chromatin prepared by using this kit can be used in a variety of chromatin immunoprecipitation methods. It is the optimal method for preparing chromatin required by One-Step ChIP Kit. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
Usage :
For research use only (RUO)
Storage :
Upon receipt: (1) Store Protease Inhibitor Cocktails at 4°C; (2) Store remaining components at room temperature.All components of the kit are stable for 6 months from the date of shipment, when stored properly.
Kit Components :
5X Lysis Buffer 2 X 20 mlExtraction Buffer A 15 mlExtraction Buffer B 15 mlExtraction Buffer C 8 mlChromatin Buffer 8 mlProtease Inhibitor Cocktail (1000X)* 15 µl* Spin the solution down to the bottom prior to use.
Compatible Sample Types :
Cell, Tissue
Features & Benefits :
Extremely fast procedure: the entire procedure from cell/tissue sample to ready-to-use chromatin is less than 2 hours.Convenient and flexible: the kit is suitable for preparing both native chromatin and cross-linked chromatin from monolayer or suspension cells, or from tissues.Unsheared chromatin makes it customizable for various analysis workflows that require either intact or fragmented chromatin, including ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, etc.
PDF