Product Overview :
Significance of Measurement: Polyphenol oxidase (PPO) is a copper-containing oxidase widely present in animals, plants, microorganisms, and cultured cells. It catalyzes the oxidation of mono- and diphenols to quinones, leading to enzymatic browning, which is closely related to fruit and vegetable processing, tea quality, and plant tissue culture.
Principle of Measurement: PPO catalyzes the oxidation of catechol to o-quinone, which exhibits a characteristic absorption peak at 410 nm. PPO activity is determined by monitoring absorbance at this wavelength.