Product Overview :
Protein Cy5 Labeling Kit provides an easy way to label proteins with Cy5 in a user-friendly spin column format. Cy5 is an ideal fluorescent dye for labeling of proteins with its intense signal in the red region of the spectrum. Each pair of spin columns provided in the kit can be used to purify up to 1 mg of the labeled target protein. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of protein per reaction. The Cy5-labeled protein has excitation and emission wavelengths at 650 nm and 670 nm respectively, and can be directly used for downstream applications including ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS, etc.
Storage :
Store the kit at 4°C, protected from light. Read the entire protocol before performing the experiment. Briefly spin small vials prior to opening. Bring the kit components to room temperature before use.
Size :
Contains sufficient reagents to label and purify 5 x 1 mg of protein
Kit Components :
Cy5, 5 vials
Spin Column, 10 columns
Elution Buffer, 10ml
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